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Monitoring and quantification on solid support of a by-product formation during peptide synthesis by Tof-SIMS

Christine Enjalbal 1 Delphine Maux 1 Gilles Subra 1 Jean N. Martinez 1 Robert Combarieu 2 Jean-Louis Aubagnac 1
1 LAPP - Laboratoire des Amino-acides Peptides et Protéines
2 CEMEF - Centre de Mise en Forme des Matériaux
Abstract : To evaluate the amount of pyroglutamic acid formation during the synthesis of glutamic acid containing peptides, resin beads were substituted by known amounts of Fmoc-Glu(O(t)Bu)-Phe-Ala-NH2 and the corresponding side- product Pyr-Phe-Ala-NH2 and directly analyzed by time of flight-secondary ion mass spectrometry. Detection and quantification of pyroglutamic acid was achieved without release of the peptide chains in solution.
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Submitted on : Wednesday, December 1, 2010 - 1:56:10 PM
Last modification on : Wednesday, November 17, 2021 - 12:28:14 PM

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CNRS | ENSMP | ENSMP_CEMEF | PARISTECH | INSTITUT-TELECOM | PSL

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Christine Enjalbal, Delphine Maux, Gilles Subra, Jean N. Martinez, Robert Combarieu, et al.. Monitoring and quantification on solid support of a by-product formation during peptide synthesis by Tof-SIMS. Tetrahedron Letters, Elsevier, 1999, 40 (34), p.6217-6220. ⟨10.1016/S0040-4039(99)01158-2⟩. ⟨hal-00541879⟩

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