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Article Dans Une Revue Journal of Photochemistry and Photobiology B: Biology Année : 2010

One pot synthesis of new hybrid versatile nanocarrier exhibiting efficient stability in biological environment for use in photodynamic therapy

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Edouard Thienot
  • Fonction : Auteur
Matthieu Germain
  • Fonction : Auteur
Kelthoum Pajos
  • Fonction : Auteur
Virginie Simon
  • Fonction : Auteur
Audrey Darmon
  • Fonction : Auteur
Julie Marill
  • Fonction : Auteur
Elsa Borghi
  • Fonction : Auteur
Laurent Levy
  • Fonction : Auteur
Agnès Pottier
  • Fonction : Auteur

Résumé

A new versatile hybrid nanocarrier has been designed using a " soft chemistry" synthesis, to efficiently encapsulate a photosensitizer - the protoporphyrin IX (Pp IX) - while preserving its activity intact in biological environment for advantageous use in photodynamic therapy (PDT). The synthesized Pp IX silica-based nanocarriers show to be spherical in shape and highly monodisperse with size extending from 10. nm up to 200. nm according to the synthesis procedure. Upon laser irradiation, the entrapped Pp IX shows to efficiently deliver reactive oxygen species (ROS) which are responsible for damaging tumor tissues. The ability of Pp IX silica-based nanocarriers to induce tumor cell death has been tested successfully in vitro. The stability of the Pp IX silica-based nanocarriers has been followed by UV-vis absorption and fluorescence emission in aqueous media and in 100% mouse serum media. The flexibility of the nanocarrier silica core has been examined as the key parameter to tune the Pp IX stability in biological environment. Indeed, an additional biocompatible inorganic surface coating performed on the Pp IX silica-based nanocarriers to produce an optimized bilayer coating demonstrates to significantly enhance the Pp IX stabilization in biological environments. Such versatile hybrid nanocarriers open new perspectives for PDT.
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Dates et versions

hal-00795388 , version 1 (28-02-2013)

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Citer

Edouard Thienot, Matthieu Germain, Kelthoum Pajos, Virginie Simon, Audrey Darmon, et al.. One pot synthesis of new hybrid versatile nanocarrier exhibiting efficient stability in biological environment for use in photodynamic therapy. Journal of Photochemistry and Photobiology B: Biology, 2010, 100 (1), pp.1-9. ⟨10.1016/j.jphotobiol.2010.03.009⟩. ⟨hal-00795388⟩
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