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Mitotic lamin disassembly is triggered by lipid-mediated signaling.

Abstract : Disassembly of the nuclear lamina is a key step during open mitosis in higher eukaryotes. The activity of several kinases, including CDK1 (cyclin-dependent kinase 1) and protein kinase C (PKC), has been shown to trigger mitotic lamin disassembly, yet their precise contributions are unclear. In this study, we develop a quantitative imaging assay to study mitotic lamin B1 disassembly in living cells. We find that CDK1 and PKC act in concert to mediate phosphorylation-dependent lamin B1 disassembly during mitosis. Using ribonucleic acid interference (RNAi), we showed that diacylglycerol (DAG)-dependent PKCs triggered rate-limiting steps of lamin disassembly. RNAi-mediated depletion or chemical inhibition of lipins, enzymes that produce DAG, delayed lamin disassembly to a similar extent as does PKC inhibition/depletion. Furthermore, the delay of lamin B1 disassembly after lipin depletion could be rescued by the addition of DAG. These findings suggest that lipins activate a PKC-dependent pathway during mitotic lamin disassembly and provide evidence for a lipid-mediated mitotic signaling event.
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Contributor : Thomas Walter Connect in order to contact the contributor
Submitted on : Friday, January 6, 2017 - 11:35:12 AM
Last modification on : Wednesday, November 17, 2021 - 12:31:04 PM


  • HAL Id : hal-01428007, version 1
  • PUBMED : 22986494


Moritz Mall, Thomas Walter, Mátyás Gorjánácz, Iain F Davidson, Thi Bach Nga Ly-Hartig, et al.. Mitotic lamin disassembly is triggered by lipid-mediated signaling.. Journal of Cell Biology, Rockefeller University Press, 2012, 198 (6), pp.981-90. ⟨hal-01428007⟩



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