A conserved choreography of mRNAs at centrosomes reveals a localization mechanism involving active polysome transport

Adham Safieddine (1) , Emeline Coleno (1) , Abdel-Meneem Traboulsi (1) , Oh Sung Kwon (2) , Frederic Lionneton (3) , Virginie Georget (3) , Marie-Cécile Robert (1) , Thierry Gostan (1) , Charles-Henri Lecellier (1) , Soha Salloum (1, 4) , Racha Chouaib (1, 4) , Xavier Pichon (1) , Hervé Le Hir (2) , Kazem Zibara (4) , Marion Peter (1) , Edouard Bertrand (1)

1 IGMM - Institut de Génétique Moléculaire de Montpellier
2 IBENS - Institut de biologie de l'ENS Paris
3 BCM - BioCampus Montpellier
4 LU / ULB - الجامعة اللبنانية [بيروت] = Lebanese University [Beirut] = Université libanaise [Beyrouth]

Local translation allows for a spatial control of gene expression. Here, we used high-throughput smFISH to screen centrosomal protein-coding genes, and we describe 8 human mRNAs accumulating at centrosomes. These mRNAs localize at different stages during cell cycle with a remarkable choreography, indicating a finely regulated translational program at centrosomes. Interestingly, drug treatments and reporter analyses revealed a common translation-dependent localization mechanism requiring the nascent protein. Using ASPM and NUMA1 as models, single mRNA and polysome imaging revealed active movements of endogenous polysomes towards the centrosome at the onset of mitosis, when these mRNAs start localizing. ASPM polysomes associate with microtubules and localize by either motor-driven transport or microtubule pulling. Remarkably, the Drosophila orthologs of the human centrosomal mRNAs also localize to centrosomes and also require translation. These data identify a conserved family of centrosomal mRNAs that localize by active polysomes transport mediated by nascent proteins.

Local translation allows for a spatial control of gene expression. Here, we used high-throughput smFISH to screen centrosomal protein-coding genes, and we describe 8 human mRNAs accumulating at centrosomes. These mRNAs localize at different stages during cell cycle with a remarkable choreography, indicating a finely regulated translational program at centrosomes. Interestingly, drug treatments and reporter analyses revealed a common translation-dependent localization mechanism requiring the nascent protein. Using ASPM and NUMA1 as models, single mRNA and polysome imaging revealed active movements of endogenous polysomes towards the centrosome at the onset of mitosis, when these mRNAs start localizing. ASPM polysomes associate with microtubules and localize by either motor-driven transport or microtubule pulling. Remarkably, the Drosophila orthologs of the human centrosomal mRNAs also localize to centrosomes and also require translation. These data identify a conserved family of centrosomal mRNAs that localize by active polysomes transport mediated by nascent proteins.

Mots clés: high-throughput screening; ASPM; smFISH; cotranslational targeting; conserved polysome choreography at centrosomes mRNA localization; local translation; centrosomes; polysome imaging; NUMA1

Domaines: Sciences du Vivant [q-bio]

Adham Safieddine, Emeline Coleno, Abdel-Meneem Traboulsi, Oh Sung Kwon, Frederic Lionneton, et al.. A conserved choreography of mRNAs at centrosomes reveals a localization mechanism involving active polysome transport. 2020. ⟨hal-03014936v1⟩